Genetics

The Department of Medical Experts was transformed into Department of Biology in 1991, which has been separated into Department of Medical Experts and of Hemogenetics, later renamed as Department of Genetics. This department carries out the comparative study of core and mithocondrial human DNA from biological remains on different exhibits for person identification following international guidelines. Based on the expert activity the Department has the duty to maintain and manage the the national forensic DNA database. The CODIS based database has complete comparability to international databases, which enables the Department to take part in international data exchange in the frame of Prüm Treaty.

Beyond the expert work the employees of the Department take part in different scientific projects, aiming the development of technology of forensic genetics.

Hamilton AutoLys STAR 4+4 robotic workstation: This freely programmable high-throughput pre-PCR instrument allows the simultaneous processing of up to 72 swabs or other simple samples from autolysis through quantification to STR-PCR set-up within 12 hours. It can be adjusted to the proper management of different preparative kits (e.g.,  PrepFiler Automated Forensic DNA Extraction Kit - Applied Biosystems, DNA IQ System Kit - Promega, etc.) as well as the most widely used STR-PCR kits (e.g., AmpFlSTR NGM SElect Amplification Kit - Applied Biosystems, PowerPlex ESI 17 Pro Systems - Promega, etc.). Sample tracebility is proven by the use of 1D and 2D barcodes.

Hamilton StarLet 4 ML robotic workstation: This freely programmable high-throughput post-PCR instrument allows the simultaneous processing of up to 25 plates within couple of  hours. It can be adjusted to the proper management of almost any kind of post-PCR sample set-up processes. Sample tracebility is proven by the use of 1D and 2D barcodes.

Applied Biosystems AutoMate Express robotic workstation: This stand alone medium-throughput pre-PCR instrument allows the simultaneous processing of up to 13 swabs or other simple samples from autolysis to DNA extract within 1-2 hours. It can be adjusted to the proper management of sometimes problematic sample types (e.g., hairs, bones, etc.). Sample tracebility is proven by the use of 1D barcodes.

Applied Biosystems 3500 xL Genetic Analyser: This high-throughput post-PCR instrument allows the simultaneous processing of up to 24 samples within 1 hour. Due to its 6-dye compatibility there is no problem with the most informative STR-PCR kits (e.g., GlobalFiler PCR Amplification Kit - Applied Biosystems, PowerPlex Fusion Systems - Promega, etc.) currently available on the market. Sample tracebility is proven by the use of  1D barcodes.

Laboratory of Reference Samples Analysis

The main function of the Laboratory of Reference Samples Analysis is to determine the DNA profiles of the convicted persons under criminal procedure and the suspects. After conducting the profiling and confirming the DNA profiles in the LIMS system, the results and documents are to be transmitted electronically to the Laboratory of DNA Database Management within the Department of Genetics. These profiles assist law enforcement agencies in identifying perpetrators, linking multiple crime scenes, and resolving missing person’s cases.

The department performs nearly 32-40 thousand DNA Profiling annually.

Furthermore, the department carries out paternity testing and makes DNA reports for the courts to assist the justice system. DNA testing provides stronger results whether they are inclusions, exclusions, or inconclusive lending to a greater confidence in the justice system.

Additionally, DNA testing has played a significant role in the future of this science due to it’s reliability, this is true justice and this is the type of justice.


Head of the Department of Genetics:

Ágota Dobos
Department of Genetics
director, forensic geneticist